A rapid and sensitive high performance liquid chromatography method for determination of paracetamol, Diclofenac sodium and Caffeine has been developed. The chromatography system used a reversed phase Hypersil 250 x 4.6 mm ODS (5 µm) column with UV- Vis detection at 230 nm. Mobile phase consisted of Phosphate Buffer pH 2.5: Acetonitrile (30: 70) (Adjust pH 2.5 with o-phosphoric acid) at a flow rate of 0.5 ml/min .The calibration curve was linear in the concentration range of 2-40 μg/ml for paracetamol, 2-32 μg/ml for Caffeine and 4-20 μg/ml for diclofenac sodium.The retention times of Paracetamol, Caffeine and Diclofenac Sodium were found to be 5.10, 5.81 and 10.38 min respectively. The results of the study showed that the proposed HPLC method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability, which is useful for the routine determination of Paracetamol, Diclofenac Sodium and Caffeine, in bulk drug and in its pharmaceutical dosage form.
Loading....